This article provides a detailed comparison of single-beam and double-beam UV-Vis spectrophotometers, specifically tailored for pharmaceutical researchers and drug development professionals. It explores the foundational principles, distinct advantages, and limitations of each instrument type. The scope extends to methodological applications in drug quantification, impurity profiling, and dissolution testing, alongside practical guidance for troubleshooting common issues and ensuring regulatory compliance. By synthesizing validation protocols and comparative performance metrics, this guide aims to equip scientists with the knowledge to select the optimal spectrophotometer for their specific pharmaceutical QA/QC and research needs, ultimately enhancing analytical accuracy and efficiency in drug development.
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